ABSTRACT
To observe and assess the performance and effect of our self-made FD-1 freezing drier on biomaterials. R502 compressor and R502 refrigerating agent were adopted. In the experiment, FD-1 lyophilized collagen sponge, strain and defibrinogenase. The evaporating-condenser temperature reached -45 degrees C and the small icebox temperature reached -30 degrees C under the loading or free-loading circumstances in the lyophilizing box. The lyophilized collagen sponge had many pores in the structure, and the strain and the defibrinogenase were lyophilized and maintained satisfactorily. This freezing drier is suitable for lyophilizing some biomaterial samples in small or medium batches.
Subject(s)
Humans , Bacteria , Collagen , Freeze Drying , Methods , TemperatureABSTRACT
Objective To investigate the feasibility of using synbiotics and probiotics to prevent and cure intestinal dysbacteriosis after burn. Methods Burned rats were fed with synbiotics and probiotics reagents, and the amounts of major intestinal florae in caecal contents were detected. Results The major physiological anaerobes were mostly stable, and the conditioned pathogens had no abnormal. Conclusion The micro-ecosystem regulator can quickly supplement the decreased physiological anaerobes caused by burning,and avoid the occurrence of dysbacteriosis.
ABSTRACT
Biological antagonism was found to be only one aspeet When we Study the interactions among the microbial populations in and on the human beings. We examined the following strains of the resident flora isolated from the human healthy skin, including propionibacterium acnes (A14-1), Staphylococcus epidermidis (F65), and a number of the common dermal pathogenic micro-organisms such as staphylococcus aurcns. Ps. acruginosa snd E. coli, for their antagonistic interactions in vitro. The following results were obtained, they revealed obvious antagonism After 48h of contact in vitro antagonistic interaction to appear, and after 72h antagonism displayed obviously. On the other hand, P. acnes (A14-1) and S. epidermidis (F65) showed no antagonisim, they behaved commesaly, such commensality seemed to be important for the autoperification of the human normal skin surfaces resulting in dermal microeubiosis of the human beings.We examined the members of the transient microbial flora of the healthy human skin Lactobacterium (ad3), Bifidobacterium (Bf1) and some common pathogenic bacteria in antagonistic experiments in vitro, and discovered that they showed micropopulations appeared to be of great value to the maintenance of good health to their host.